Immunoprecipitating PD-L2 in Hela whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA017667A0m in Hela whole cell lysate. Lane 2: CSB-MA017667A0m (2-¦l) + Hela whole cell lysate (500-¦g)
Lane 3: Hela whole cell lysate (20-¦g)
For western blotting, the blot was detected with CSB-MA017667A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

Western Blot
Positive WB detected in: Hela whole cell lysate at 20++g, 10++g, 5++g, 2.5++g, 1.25++g All lanes: PD-L2 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31,21 KDa
Observed band size: 45-50 KDa
Exposure timen+Ü5min

IHC image of CSB-MA017667A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4-¦C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4-¦C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).